Model‐free quantification and visualization of colocalization in fluorescence images
نویسندگان
چکیده
منابع مشابه
Model-Based Segmentation and Colocalization Quantification in 3D Microscopy Images
We introduce a new model-based approach for automatic quantification of colocalizations in 3D microscopy images. The approach is based on different 3D parametric intensity models in conjunction with a model fitting scheme to quantify subcellular structures with high accuracy. The central idea is to determine colocalizations between different channels based on the estimated geometry of subcellul...
متن کاملQuantitative colocalization analysis of confocal fluorescence microscopy images.
Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of images with colocalization based on the calculation of a number of specialized coefficients. First, images of double-stained sections are subjected to background correction. Then, various coefficients are calculated. Meanings of the coefficients and a guide to in...
متن کاملColocalization analysis in fluorescence microscopy.
The measurement of colocalization requires images of two fluorophores that are aligned, with no cross talk, and that the intensities remain within the response range of the microscope. Quantitation depends upon differentiating between the presence and absence of fluorescence, and measurements should be made within biologically relevant regions of interest. Co-occurrence can be measured simply b...
متن کاملColocalization of fluorescence and Raman microscopic images for the identification of subcellular compartments: a validation study.
A major promise of Raman microscopy is the label-free detailed recognition of cellular and subcellular structures. To this end, identifying colocalization patterns between Raman spectral images and fluorescence microscopic images is a key step to annotate subcellular components in Raman spectroscopic images. While existing approaches to resolve subcellular structures are based on fluorescence l...
متن کاملA Hybrid Method for Segmentation and Visualization of Teeth in Multi-Slice CT scan Images
Introduction: Various computer assisted medical procedures such as dental implant, orthodontic planning, face, jaw and cosmetic surgeries require automatic quantification and volumetric visualization of teeth. In this regard, segmentation is a major step. Material and Methods: In this paper, inspired by our previous experiences and considering the anatomical knowledge of teeth and jaws, we prop...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2018
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.23356